atp assay kit  (MedChemExpress)

Journal: Poultry Science

Article Title: Natural astaxanthin enhances testosterone synthesis by improving mitochondrial function and reducing oxidative stress in Leydig cells

doi: 10.1016/j.psj.2026.106663

Figure Lengend Snippet: Effects of ASTA on MMP, ATP content, relative mtDNA Copy Number and mitochondrial biogenesis-related proteins in rooster Leydig cells. (A) Flow cytometry analysis of mitochondrial membrane potential. (B) Quantification of MMP (% of JC-1 red fluorescence). (C) ATP content in Leydig cells. (D) Relative mtDNA Copy Number. The relative mRNA levels of PGC-1α (E), NRF1 (F), TFAM (G). The relative protein levels of PGC-1α(H, I), NRF1(H, J), TFAM(H, K). The data represent the mean ± SEM; n = 3 in each group. * P < 0.05; ** P < 0.01.

Article Snippet: MMP, cellular ATP levels, and apoptosis in Leydig cells were assessed using the following commercial kits: the JC-1 Mitochondrial Membrane Potential Assay Kit (BiYunTian, China), the ATP Content Assay Kit (Nanjing Jiancheng Bioengineering Institute, China), TIANamp Genomic DNA Kit (Tiangen, Beijing, China) and the Annexin V-FITC Apoptosis Detection Kit (BiYunTian, China), in conjunction with a flow cytometer.

Techniques: Flow Cytometry, Membrane, Fluorescence